[Question] Can I perform ANCOVA on a model with 3 independent variables?

I have 4 variables:

Dependent variable: Feo

Independent variables: Ni & Fe & Categorical factor (hot vs cold)

Feo is a function of Ni & Fe. I want to determine if Feo is affected by hot vs cold (prediction is that Feo is higher if hot).

pretty much this but with an additional independent variable:

http://r-eco-evo.blogspot.com/2011/08/comparing-two-regression-slopes-by.html

can it be done or because there are too many variables it’s hard to determine the effect of cold vs cold?

also, best software/language to use ?

Thanks

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Nevin Manimala

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