Help with analyzing results from research project in order to minimize replication

Hello all, I’m an undergrad and have been interning for the last 6+ months monitoring bacteria quantity in a number of stream sites.

As a little background for each site I test a 1ml and .1ml amount of water and analyze bacteria quantity, I do each site in triplicate amounting to 6 total per sample (1ml x3, .1ml x3). I’m looking to minimize replication (to save money, time etc) ideally I’d like to only replicate in twice. What kind of analysis can I do to my data to see if the 3rd replication is not much different from the rest?

Sorry for grammar/typos on mobile.

Thanks!

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Nevin Manimala

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